Coding

Part:BBa_K2871004:Design

Designed by: Nattawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-10-07)


CesT T3SS substrate chaperone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CesF and CesT chaperone gene is present in a genomic region called 'locus of enterocyte effacement' (LEE) of Enteropathogenic E. coli. We choose to order codon-optimized synthetic gene from IDT to eliminate most common restriction sites.


Source

Amino acid sequence retrieved from Enteropathogenic E. coli (EPEC) O127:H6 strain E2348/69. Codon-optimized and synthesized by IDT.

References